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Friday, 02.12.2016
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Kro I: an online article PDF Print E-mail
Wednesday, 29 February 2012 00:00

We have made the new version of  the online article "A new methyl-directed site-specific endonuclease KroI recognizes and cuts DNA sequence 5’-G^C(5mC)GGC-3’". Now the full text you can read in "the last articles" section or directly via this link.

 
The new Methyl-directed endonuclease - Pkr I PDF Print E-mail
Wednesday, 15 February 2012 10:00

We are pleased to announce a new methyl-dependent restriction endonuclease - Pkr I.
Detailed specifications and other additional information you can see on the info-page of this enzyme.

MD Tools were updated to use this endonuclease recognition site.

 

 

 

 

 

 

 
We have changed our way! PDF Print E-mail
Written by VicoNT   
Sunday, 12 February 2012 09:00

The MeBase site has changed! Today our site has changed the look, navigation and content. In the near future there will be new tools and features. We will also continue to publish all available information concerning the methyl-dependent restriction endonucleases.

 

 

 
The last 5 articles PDF Print E-mail
Wednesday, 15 February 2012 09:00

Now on Mebase you can easily read our latest five articles dealing with methyl-directed endonucleases. They are always available in section "Key Concepts" as a "The last Articles ".

 

 

 

 

 

 

 

 

 
The new article in our Online library PDF Print E-mail
Friday, 20 January 2012 00:00

We have published the new article "Epigenetic typing of human cancer cell lines by BlsI- and GlaI-PCR assays". Short summary:

 

BlsI- and GlaI-PCR assays have been applied to determine a methylation status of regulatory regions of SEPT9b, IGFBP3, CEBPD, MGMT and RASSF1A tumor suppressor genes in malignant cell lines HeLa, Raji, U-937, Jurkat and in the control fibroblast cell line L-68. GlaI- and BlsI-PCR assays have shown either presence or absence of 5’-R(5mC)GY-3’ sites in the regulatory regions of the studied tumor suppressor genes depending on malignant cell line, which was a source of DNA preparation. Regulatory regions are methylated in different combinations in the studied malignant cell lines. At the same time no 5’-R(5mC)GY-3’ sites have been found in these regulatory regions in DNA from normal fibroblast cell line L-68. These results show that method of BlsI- and GlaI-PCR assays allow to determine and to discriminate malignant cell lines. Thus, BlsI- and GlaI-PCR assays may be used for epigenetic typing of malignant cell lines.

 

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